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The substrate Alternative was then saved at −twenty°C until finally the ammonium and nitrate contents ended up analyzed While using the San++ Constant-Move Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate willpower was dependant on a reaction with hydrazinium sulfate, sulfanilamide and N-(one-naphthyl) ethylenediamine dihydrochlorid
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